Endocrine Abstracts

Increasing evidences indicate that brown adipose tissue (BAT) functional activity is reduced during obesity. The nature of this deficit in BAT function, however, is still poorly understood. To analyze whether insulin function in the BAT is altered during obesity, we studied the in vivo metabolic activity of BAT by PET/CT imaging and euglycaemic hyperinsulinemic clamp in lean and diet-induced obese mice. Three groups of mice were analyzed after administration of different diet ...

Introduction: The isoform A of the human insulin receptor (IR-A) binds insulin with high affinity, and binds IGF-II with a 3–10 folds lower affinity. Cells lacking the insulin-like Growth Factor-I receptor (IGF-IR) and overexpressing the human IR-A (R-/IR-A cells) respond to IGF-II with reduced metabolic effects but unaltered or increased mitogenesis as compared to insulin stimulation. We hypothesized that this altered ratio of metabolic-to-mitogenic effects of IGF-II in ...

Brown adipose tissue (BAT) is an insulin sensitive organ having a very high uptake of glucose per gram of tissue. The role of insulin on glucose uptake in BAT is still poorly understood, moreover, it is not clear whether BAT insulin function is compromised with obesity. We used a small animal PET/CT imaging approach to analyse insulin function in vivo in lean and obese mice using a glucose analogue PET tracer (18F-FDG). Three groups of mice were analysed afte...